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Using a novel genetic clock, a team of researchers from Kiel, London, Oldenburg, and Davis, California, has determined the age of a large marine plant clone for the first time. This seagrass clone from the Baltic Sea dates back to the migration period 1,400 years ago. The newly developed clock can be applied to many other species, from corals and algae to plants such as reeds or raspberries. The scientists have published their work in the journal Nature Ecology and Evolution.

“Vegetative reproduction as an alternative mode of reproduction is widespread in the animal, fungal, and plant kingdoms,” explains research leader Dr. Thorsten Reusch, Professor of Marine Ecology at the GEOMAR Helmholtz Centre for Ocean Research Kiel. These so-called “clonal species” produce genetically similar offspring by branching or budding and often reach the size of a football field or more. However, these offspring are not genetically identical.

Dynamics of SoGV in generic clonal organisms.

(a), Multicellular clonal species exist across the tree of life. 

(b), Allele frequency change of SoGV due to the formation of new modules by branching or splitting. A new module is initiated either directly by the stem cells (that is, splitting) or by the daughter cells of the stem cells (that is, branching). Splitting reduces the size of the original stem cell population, while branching leaves the original cell population untouched. During the formation of new modules, the cell population undergoes a genetic bottleneck. c,d, The accumulation rate of fixed SoGV is independent of module formation rate. The tree topology depicts a module undergoing (multiple) module formation events, where the dashed line and the solid line represent the original module and the new module respectively. New mutations (M) occur at a constant rate, and only mutations in the new modules are depicted (with a different colour). For each timepoint, the vertical length of the colours represents the frequency of the SoGV within the module. Clonal dynamics in a single module (solid line in tree structure) are depicted as a Muller plot that shows the nested allele frequency of SoGV over time. The frequency of SoGV changes during module formation events, due to the bottleneck. Eventually, SoGVs are either fixed or lost. Under low module formation rate

(c), fixation events are rare. Thus, many SoGVs have accumulated in the intervening time and are fixed simultaneously. Under high module formation rate

(d), fixation events occur more frequently, but with fewer SoGVs fixed at each branching event. 

CREDIT: Nature Ecology & Evolution (2024). DOI: 10.1038/s41559-024-02439-z

Previous work by a team led by GEOMAR researchers had already shown that somatic mutations accumulate in vegetative offspring, a process similar to cancer. Now, a team led by Prof. Dr. Reusch, Dr. Benjamin Werner (Queen Mary University London, QMUL), and Prof. Dr. Iliana Baums (Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg, HIFMB) has used this mutation accumulation process to develop a novel molecular clock that can determine the age of any clone with high precision.

Researchers at the University of Kiel, led by Professor Reusch, applied this novel clock to a worldwide dataset of the widespread seagrass Zostera marina (eelgrass), ranging from the Pacific to the Atlantic and the Mediterranean.

In Northern Europe in particular, the team found clones with ages of several hundred years, comparable to the age of large oak trees. The oldest clone identified was 1402 years old and came from the Baltic Sea. This clone reached this advanced age despite a harsh and variable environment. This makes the eelgrass clone older than the Greenland shark or the Ocean Quahog, which live only a few hundred years.

These new age and longevity estimates for clonal species fill an important knowledge gap. Particularly in marine habitats, many fundamental habitat-forming species such as corals and seagrasses can reproduce vegetatively, and their clones can become very large. The continuous production of small, genetically identical but physically separated shoots or fragments from the parent clone means that age and size are decoupled in these species.

The new study now provides a tool to date these clones with high accuracy. “Such data are, in turn, a prerequisite for solving one of the long-standing puzzles in conservation genetics, namely why such large clones can persist despite variable and dynamic environments,” says Reusch.

Once a high-quality eelgrass genome was available, work could begin. Another key factor in the study was that colleagues at the University of California, Davis (UC Davis) had kept a seagrass clone in their culture tanks for 17 years, which served as a calibration point.

“This paper shows how interdisciplinary interactions between cancer evolutionary biologists and marine ecologists can lead to new insights,” says Dr. Werner, Lecturer in Mathematics and Cancer Evolution at QMUL, who focuses on the somatic evolution of tumors which also develop clonally.

Prof. Dr. Baums, molecular ecologist at the HIFMB, adds, “We can now apply these tools to endangered corals to develop more effective conservation measures, which we urgently need as unprecedented heat waves threaten coral reefs.”

“We expect that other seagrass species and their clones of the genus Posidonia, which extend over more than ten kilometers, will show even higher ages and thus be by far the oldest organisms on Earth,” says Reusch. These will be the next objects of study.

More information:  Lei Yu et al, A somatic genetic clock for clonal species, Nature Ecology & Evolution (2024). DOI: 10.1038/s41559-024-02439-z

This article is republished from PHYS.ORG and provided by Helmholtz Association of German Research Centres